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| Bunetel, L; Segui, A; Guerin, J; Lerman, I; Cormier, M. |
| The principle of this method is to evaluate, by counts exercised upon selective media, the relative importance of the different bacteria families in the flora of the coastal waters. It is important to separate the contaminant species and the commensal species in the marine environment. Ten culture media distributed into four categories (selective, non selective, saline and non saline) were examined by numeration. Counts were transferred on a diagram allowing to plot down a profile of bacterial flora of water sampled from a given geographical area. The profiles are arranged by a classification method. The sampling points with similar or adjoining profiles were assembled and marked on the chart. In this way, the authors obtained areas representing the impact... |
| Tipo: Text |
Palavras-chave: Bacteria; Coastal waters; Microbiological culture; Sewage disposal; Microbial contamination; Bacterial counters; Bacteria collecting devices. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-1004.pdf |
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| Bruni, V; Crisafi, E; Maugeri, T; Zaccone, R. |
| 110 Vibrio strains were isolated, at various times of the year, from samples of sea-water supply, breeding-tank water and discarded fish used as food at the breeding stations. From the results obtained by the biochemical and cultural tests, it was possible to identify most of the isolated strains as Vibrio anguillarum . Strains with intermediate characteristics, above-all positive saccharose, proved to be numerous. The authors discuss the occurrence, in particular environments such as those being studied, of atypical or intermediate vibrio strains which are difficult to classify. |
| Tipo: Text |
Palavras-chave: Vibrio; Microbiological culture; Bacteria; Food; Minced products; Fish ponds; Sea water; Identification; Marine aquaculture; Bacteria collecting devices; Microbiological analysis. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-997.pdf |
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| White, D. |
| Microbes in nature are often concentrated on surfaces where they exist in microcolonies of mixed composition. The assay of these mixed microbial consortia by utilizing biochemical components eliminates the problems associated with cultural selectivity of viable counts or the requirement for quantitative release from surfaces required in some microscopic techniques. The assay of cellular components restricted to subsets of the microbial community gives insights into the community structure of the biofilm whereas assay of cellular components common to all cells can be used for biomass estimations. Formation of endogenous storage materials can be utilized as measures of nutritional status and rates of formation or turnover of specific "signatures" from... |
| Tipo: Text |
Palavras-chave: Bacteria; Fouling; Bioassays; Metabolism; Surfaces; Bacteria collecting devices; Culture media; Microbiological analysis. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-963.pdf |
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| Okpokwasili, G; Somerville, C; Grimes, D; Colwell, R. |
| A differential, phenanthrene-enrichment agar plating technique was used to isolate phenanthrene-degrading bacteria from phenanthrene-enriched Chesapeake Bay sediment. One of the isolates, a yellow pigmented, slime-producing, Gram-negative rod identified as Flavobacterium sp. has been studied in detail. It has been found to carry a single plasmid with a mass of about 34 megadaltons. Results of hydrocarbon adherence tests showed that the organism adhered only minimally to n-octane and n-hexadecane, but emulsified cyclohexylbenzene and 1,2,3,4,-tetrahydronaphthalene (tetralin). Curing of the plasmid with 3 mu g/ml novobiocin resulted in loss of phenanthrene clearing ability. Ability to degrade phenanthrene was confirmed using super(14)C-labeled phenanthrene. |
| Tipo: Text |
Palavras-chave: Flavobacterium; Microbiological culture; Culture media; Bacteria; Sediment pollution; Biodegradation; Bacteria collecting devices. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-1014.pdf |
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